Hormones and pregnancy.
Conversely, if a bitch aborts due to an infection or some trauma etc., the progesterone level will often fall rapidly.
To summarize, assessing the progesterone level during pregnancy diagnosis will it possible to determine whether a bitch is unfertilized or whether she has aborted.
The placenta permits exchanges between mother and the unborn litter from the time of conception until they are born. The placenta forms a circular band around the foetus (the so-called zonary placenta) . Its dark green pigment is the reason for the characteristic colour of the first discharges hen whelping. It is interesting to note that it secrets a hormone, relaxin, which can be used for diagnosing pregnancy.
The unborn puppy is wrapped in several pockets. The first, the amnion, is filled with a amniotic fluid. It acts as a shock-absorber, has a nutrient effect and plays a detoxifying role. The second, which surrounds the amnion, is the allantois. This is the well known “water sac” which is pierced before the bitch whelps.
The fertilization of the ovum by a spermatozoon forms an egg that must undergo several divisions and travel to the uterus before becoming implanted in the uterine mucosa. In bitches, the implantation does not incur until 17 days after fertilization, and results in the formation of embryonic vesicles that cannot be detected by ultrasound until the third week of pregnancy (18 days at the earliest).
After the third week, careful palpation of the
abdomen can reveal beading of the uterus, if the bitch is not too fat and
the abdominal wall is relaxed. Between the fifth and the sixth weeks, the
diameter of the uterus reaches the size of an intestinal loop. During
this period, it is difficult to distinguish between a gravid uterus and a
loop containing hard stools.
There is no point in taking X-rays until the end of
pregnancy, since the foetus’ skeleton does not become calcified (and
thus, opaque to X-rays) until after the 45th day.
techniques for diagnosing pregnancy occur too late or are too uncertain
to be reliable. These include behavioural changes, detection of foetal
heartbeats (audible in some bitches during the last two weeks of
pregnancy) by auscultation, changes in the blood (sedimentation rate,
hematocrit), or mammary development.
the earliest diagnosis of pregnancy is provided by ultrasound.
There is also a blood test based on the production of relaxin by the placenta which is thus only present during pregnancy. This hormone begins to b noticeable towards the twentieth day, but it is preferable to wait until the twenty fifth day to increase the reliability of the test. This test can be carried out as early as ultrasound, but does not give any indication of the size of the litter or of the stage of pregnancy reached.
Gestation in dogs lasts from 58 to 68 days (63 days on average). The differences observed among bitches are linked to the difference between the mating date and the actual date fertilization.
In fact, spermatozoa can live for up to five days
in the female genital tract until the ova become fertile.
After fertilization, the eggs become embryos, which
travel from the oviducts to the uterus and space themselves out through
the two uterus horns. Implantation of the embryo in the uterine mucosa
does not occur until sometime between the 17th and 19th
days after fertilization, so confirmation of pregnancy by ultrasound is
impossible before that time.
Transformation of the embryo into a foetus, and subsequent foetal growth, are possible thanks to nutrients brought by the placenta and to the development of other membranes (the amnion and the allantois) that surround and protect the foetus.
Foetal growth does not become externally visible until the second half of pregnancy.
( with permission copied from the Royal Canin Encyclopaedia/Aniwa publishing)